Engineering stereoselectivity of ThDP-dependent enzymes
نویسندگان
چکیده
منابع مشابه
Influence of Organic Solvents on Enzymatic Asymmetric Carboligations
The asymmetric mixed carboligation of aldehydes with thiamine diphosphate (ThDP)-dependent enzymes is an excellent example where activity as well as changes in chemo- and stereoselectivity can be followed sensitively. To elucidate the influence of organic additives in enzymatic carboligation reactions of mixed 2-hydroxy ketones, we present a comparative study of six ThDP-dependent enzymes in 13...
متن کاملEngineering enzymes for biocatalysis.
Protein engineering techniques have been available for over two decades beginning with the development of methods for genetic engineering. Since that time, the engineering of enzymes has advanced rapidly along with a revolution in the range and efficiency of new techniques and strategies for designing and evolving proteins in the laboratory. While recent advances in high-throughput screening te...
متن کاملPhosphorylation of carbovir enantiomers by cellular enzymes determines the stereoselectivity of antiviral activity.
Two enantiomers of carbovir, a carbocyclic analog of 2',3'-dideoxyguanosine, were compared with respect to their phosphorylation and the phosphorylation of their nucleotides by mammalian enzymes. 5'-Nucleotidase catalyzed the phosphorylation of (-)-carbovir, which is active against HIV (human immunodeficiency virus), but did not phosphorylate (+)-carbovir. (-)-Carbovir monophosphate was 7,000 t...
متن کاملPLP-Dependent Enzymes
The main focus of this special issue is on structural, functional, and biomedical studies on pyridoxal-5-phosphate(PLP-) dependent enzymes. The unparalleled catalytic versatility of PLP, the active form of vitamin B6, originates from its unique electron-sinking properties, which stabilize reaction intermediates, thus lowering the activation barrier during catalysis. At least five different prot...
متن کاملEngineering of Enzymes for Selective Catalysis
Naturally occurring enzymes are truly remarkable catalysts. However, many are not suitable for practical synthetic chemistry because of poor substrate or product selectivity. This review highlights recent advances in engineering enzymes for selective catalysis. Selected topics include altering substrate specificity, altering substrate and product selectivity, engineering enzymes that catalyze c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Journal
سال: 2013
ISSN: 1742-464X
DOI: 10.1111/febs.12496